1940’s – Pap Testing
1954 – Atlas of Exfoliative Cytology
George N. Papanicolaou
Conventional Pap smears
The conventional Pap test is certainly the most cost-effective cancer reduction program ever created. The first cervical cancer screening institutions were established in the 1940s after the important discovery of George N. Papanicolaou, who gave his name to the staining protocol that is still the international standard. This staining protocol is applied to cells collected from the cervix using a spatula, or using a brush in the case of modern Liquid Based Cytology (LBC).